KitComponents
RiboM7Gcapanalog(10mM)
T7RNApolymeraseMixture
RNase-freeDNaseI
10XTranscriptionbuffer
3MNaOACpH@5.0
Nuclease-freewater
Maxicappingtube
UserManual
MaxCappingtubesmustbestoredat4°Candstableformorethan6months
Othercomponentsmustbekeptat-20°Cfreezer
RelatedProducts
MaxCappingtubes
M-7GCapanalog
RNAclean-upkit
SuperRNATranscription
Gentlytapthereactiontubeevery15minduringthetranscriptionreaction.
BeforeprecipitatingtranscribedRNAs,transferallthereactionintoanewtube.
DoyouknowhowmanymicrogramcappedmRNAcanbemadeina20µlofinvitrotranscriptionreaction?
Ifusingthetranscriptionkitsfromotherthebiotechcompanies,thetotalyieldofcappedmRNAsina20µltanscriptionis~2-3µg,withacappingefficiency~80%.
InNeuBiogene'scappingkits,aspecialtranscriptionenhancerwasaddedinthetranscriptiontubesothatthetotalyieldofcappedmRNAcanreach>12-15µgina20µloftranscriptionreaction.Also,thecappingefficiencyis>90%.
ByusingNeuBiogene'smaxcappingtranscriptionsystem,moretargetproteinscanbetranslatedintissuecells,suchasfrogxenopusembryosorculturedcells.